產品詳情
簡單介紹：
重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白與其它公司提供的重組蛋白不同，rHuMIP-3/CCL23蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白
| Synonyms | CKB-8, MIP-3, MIPIF-1 |
| Species | Human |
| Accession | P55773 |
| GeneID | 6368 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白Approximately 11.4 kDa, a single, non-glycosylated polypeptide chain containing 99 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMIP-3/CCL23 as determined by LAL method. |
| Reconstitution | 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類巨噬細胞炎性蛋白質3 / CCL23蛋白 | |
| SDS-PAGE |
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| Reference |
1. Novak H, Muller A, Harrer N, et al. 2007. J Immunol, 178: 4335-41. 2. Votta BJ, White JR, Dodds RA, et al. 2000. J Cell Physiol, 183: 196-207. 3. Poposki JA, Uzzaman A, Nagarkar DR, et al. 2011. J Allergy Clin Immunol, 128: 73-81 e4. |
| Background | CCL23 is a member of CC chemokine family and is encoded by CCL23 gene located on Chr.17 in humans, where near several other CC chemokines. Highly expressed in ***** lung, liver, skeletal muscle and pancreas, this protein shows strong chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. It elicits the effects by binding to CCR1. CCL23 is reported that it can be cleaved into four forms: CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99). |
