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產品名稱：重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白
產品型號：rHuMIP-1β/CCL4
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白與其它公司提供的重組蛋白不同，rHuMIP-1β/CCL4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白

Synonyms G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2

Species Human

Accession P13236

GeneID 6351

Source 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白Escherichia coli.

Molecular Weight Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuMIP-1β/CCL4 as determined by LAL method.

Reconstitution 重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.

重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白

Reference 1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.
2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.
3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5.
4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52.

Background CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.

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Synonyms	G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
Species	Human
Accession	P13236
GeneID	6351
Source	重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白Escherichia coli.
Molecular Weight	Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuMIP-1β/CCL4 as determined by LAL method.
Reconstitution	重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白
Reference	1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70. 2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5. 3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5. 4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52.
Background	CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.

產品名稱：重組人巨噬細胞炎癥蛋白-1 beta / CCL4蛋白