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產品名稱：重組人類單核細胞趨化蛋白-2 / CCL8蛋白
產品型號：rHuMCP-2/CCL8
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人類單核細胞趨化蛋白-2 / CCL8蛋白與其它公司提供的重組蛋白不同，rHuMCP-2/CCL8蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類單核細胞趨化蛋白-2 / CCL8蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類單核細胞趨化蛋白-2 / CCL8蛋白

Synonyms HC14, Small-inducible cytokine A8

Species Human

Accession P80075

GeneID 6355

Source Escherichia coli.

Molecular Weight 重組人類單核細胞趨化蛋白-2 / CCL8蛋白Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance 重組人類單核細胞趨化蛋白-2 / CCL8蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組人類單核細胞趨化蛋白-2 / CCL8蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類單核細胞趨化蛋白-2 / CCL8蛋白

SDS-PAGE

Reference 1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31.
2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.
3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74.
4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92.

Background Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

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Synonyms	HC14, Small-inducible cytokine A8
Species	Human
Accession	P80075
GeneID	6355
Source	Escherichia coli.
Molecular Weight	重組人類單核細胞趨化蛋白-2 / CCL8蛋白Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	重組人類單核細胞趨化蛋白-2 / CCL8蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組人類單核細胞趨化蛋白-2 / CCL8蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類單核細胞趨化蛋白-2 / CCL8蛋白
SDS-PAGE
Reference	1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31. 2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42. 3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74. 4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92.
Background	Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

產品名稱：重組人類單核細胞趨化蛋白-2 / CCL8蛋白