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產品名稱：重組人類生長調節蛋白-γ/ CXCL3蛋白
產品型號：rHuGRO-γ/CXCL3
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人類生長調節蛋白-γ/ CXCL3蛋白與其它公司提供的重組蛋白不同，rHuGRO-γ/CXCL3蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類生長調節蛋白-γ/ CXCL3蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類生長調節蛋白-γ/ CXCL3蛋白

Synonyms GRO-gamma, MIP2-beta

Species Human

Accession P19876

GeneID 2921

Source Escherichia coli.

Molecular Weight 重組人類生長調節蛋白-γ/ CXCL3蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人類生長調節蛋白-γ/ CXCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuGRO-γ/CXCL3 as determined by LAL method.

Reconstitution 重組人類生長調節蛋白-γ/ CXCL3蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類生長調節蛋白-γ/ CXCL3蛋白

SDS-PAGE

Reference 1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.
2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.
3. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.

Background CXCL3, also named GRO-γ, is belonging to the CXC chemokine family and encoded by the gene CXCL3. CXCL3/GRO-γ shares 86 % amino acid sequence with CXCL1/GRO-α. All three human GROs (GRO-α, GRO-β, GRO-γ) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three GROs can bind with high affinity to the IL-8 receptor type B.

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Synonyms	GRO-gamma, MIP2-beta
Species	Human
Accession	P19876
GeneID	2921
Source	Escherichia coli.
Molecular Weight	重組人類生長調節蛋白-γ/ CXCL3蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人類生長調節蛋白-γ/ CXCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuGRO-γ/CXCL3 as determined by LAL method.
Reconstitution	重組人類生長調節蛋白-γ/ CXCL3蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類生長調節蛋白-γ/ CXCL3蛋白
SDS-PAGE
Reference	1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6. 2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83. 3. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84. 4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
Background	CXCL3, also named GRO-γ, is belonging to the CXC chemokine family and encoded by the gene CXCL3. CXCL3/GRO-γ shares 86 % amino acid sequence with CXCL1/GRO-α. All three human GROs (GRO-α, GRO-β, GRO-γ) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three GROs can bind with high affinity to the IL-8 receptor type B.

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產品名稱：重組人類生長調節蛋白-γ/ CXCL3蛋白