產品詳情
簡單介紹：
重組鼠血小板因子-4 / CXCL4蛋白與其它公司提供的重組蛋白不同，rMuPF-4/CXCL4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠血小板因子-4 / CXCL4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠血小板因子-4 / CXCL4蛋白
| Species | Murine |
| Accession | Q9Z126 |
| GeneID | 56744 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠血小板因子-4 / CXCL4蛋白Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠血小板因子-4 / CXCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method. |
| Reconstitution | 重組鼠血小板因子-4 / CXCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠血小板因子-4 / CXCL4蛋白 | |
| Reference |
1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44. 3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49. 4. Warkentin TE. 2007. N Engl J Med. 356:891-3. |
| Background | CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity. |
