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產品名稱：重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白
產品型號：rMuMDC/CCL22
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白與其它公司提供的重組蛋白不同，rMuMDC/CCL22蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白

Synonyms STCP-1, MDC (1-69), Macrophage-derived Chemokine

Species Murine

Accession O88430

GeneID 20299

Source Escherichia coli.

Molecular Weight 重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human activated lymphocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rMuMDC/CCL22 as determined by LAL method.

Reconstitution 重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白

SDS-PAGE

Reference 1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1.
2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604.
3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14.
4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.

Background CCL22 is a protein that in mouse is encoded by the CCL22 gene, which locates on the Chr. 8. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).

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Synonyms	STCP-1, MDC (1-69), Macrophage-derived Chemokine
Species	Murine
Accession	O88430
GeneID	20299
Source	Escherichia coli.
Molecular Weight	重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human activated lymphocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rMuMDC/CCL22 as determined by LAL method.
Reconstitution	重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白
SDS-PAGE
Reference	1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1. 2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604. 3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14. 4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.
Background	CCL22 is a protein that in mouse is encoded by the CCL22 gene, which locates on the Chr. 8. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).

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產品名稱：重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白