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產品名稱：重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白
產品型號：rRtTARC/CCL17
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白與其它公司提供的重組蛋白不同，rRtTARC/CCL17蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白

Species Rat

Accession Q9ERE0

GeneID 117518

Source Escherichia coli.

Molecular Weight 重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin 重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白Less than 0.1 EU/μg of r rRtTARC/CCL17 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白

SDS-PAGE

Reference 1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308.
2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21.
3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21.
4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8.
5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.

Background CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.

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Species	Rat
Accession	Q9ERE0
GeneID	117518
Source	Escherichia coli.
Molecular Weight	重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白Less than 0.1 EU/μg of r rRtTARC/CCL17 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白
SDS-PAGE
Reference	1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308. 2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21. 3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21. 4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8. 5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.
Background	CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.

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產品名稱：重組大鼠胸腺和激活調節的趨化因子/ CCL17蛋白