產品詳情
簡單介紹：
重組人胰島素樣生長因子- 1蛋白與其它公司提供的重組蛋白不同，rHuIGF-1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人胰島素樣生長因子- 1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人胰島素樣生長因子- 1蛋白
| Synonyms | Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF |
| Species | Human |
| Accession | 重組人胰島素樣生長因子- 1蛋白P05019 |
| GeneID | 3479 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Quantity | 100μg/500μg/1000μg |
| AA Sequence | 重組人胰島素樣生長因子- 1蛋白GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg. Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Physical Appearance | 重組人胰島素樣生長因子- 1蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
| Endotoxin | Less than 0.01 EU/μg of rHuIGF-1 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable for 3 years at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to two weeks at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人胰島素樣生長因子- 1蛋白 | |
| SDS-PAGE |
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