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產(chǎn)品名稱(chēng)：重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白
產(chǎn)品型號(hào)：rHuTIGAR-TAT
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白與其它公司提供的重組蛋白不同，rHuTIGAR-TAT蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.

詳情介紹：

重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白

Species Human

Accession Q9NQ88

GeneID 57103

Source Escherichia coli.

Molecular Weight 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白Approximately 31.7 kDa, a single non-glycosylated polypeptide chain containing 283 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA.

Endotoxin Less than 0.1 EU/μg of rHuTIGAR-TAT as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白

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Species	Human
Accession	Q9NQ88
GeneID	57103
Source	Escherichia coli.
Molecular Weight	重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白Approximately 31.7 kDa, a single non-glycosylated polypeptide chain containing 283 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA.
Endotoxin	Less than 0.1 EU/μg of rHuTIGAR-TAT as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白

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產(chǎn)品名稱(chēng)：重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白