產(chǎn)品詳情
簡(jiǎn)單介紹：
重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白與其它公司提供的重組蛋白不同，rHuTIGAR-TAT蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白
| Species | Human |
| Accession | Q9NQ88 |
| GeneID | 57103 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白Approximately 31.7 kDa, a single non-glycosylated polypeptide chain containing 283 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA. |
| Endotoxin | Less than 0.1 EU/μg of rHuTIGAR-TAT as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類(lèi)TP53-induced糖酵解和細(xì)胞凋亡Regulator-TAT蛋白 |
