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產(chǎn)品名稱：重組人Neuregulin 1αEGF域蛋白
產(chǎn)品型號：rHuNRG1-α
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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簡單介紹：

重組人Neuregulin 1αEGF域蛋白與其它公司提供的重組蛋白不同，rHuNRG1-α蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人Neuregulin 1αEGF域蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人Neuregulin 1αEGF域蛋白

Species Human

Accession Q02297

GeneID 3084

Source Escherichia coli.

Molecular Weight 重組人Neuregulin 1αEGF域蛋白Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids.

Quantity 10μg/50μg/1000μg

AA Sequence SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人Neuregulin 1αEGF域蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of ＞ 2.5 × 10⁴ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 6.0, 150 mM NaCl.

Endotoxin Less than 0.1 EU/μg of rHuNRG1-α as determined by LAL method.

Reconstitution 重組人Neuregulin 1αEGF域蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人Neuregulin 1αEGF域蛋白

SDS-PAGE

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Species	Human
Accession	Q02297
GeneID	3084
Source	Escherichia coli.
Molecular Weight	重組人Neuregulin 1αEGF域蛋白Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids.
Quantity	10μg/50μg/1000μg
AA Sequence	SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人Neuregulin 1αEGF域蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of ＞ 2.5 × 10⁴ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 6.0, 150 mM NaCl.
Endotoxin	Less than 0.1 EU/μg of rHuNRG1-α as determined by LAL method.
Reconstitution	重組人Neuregulin 1αEGF域蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人Neuregulin 1αEGF域蛋白
SDS-PAGE

產(chǎn)品名稱：重組人Neuregulin 1αEGF域蛋白