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產品名稱：重組人胸腺和激活規范趨化因子/ CCL17蛋白
產品型號：rHuTARC/CCL17
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人胸腺和激活規范趨化因子/ CCL17蛋白與其它公司提供的重組蛋白不同，rHuTARC/CCL17蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人胸腺和激活規范趨化因子/ CCL17蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人胸腺和激活規范趨化因子/ CCL17蛋白

Species Human

Accession Q92583

GeneID 6361

Source Escherichia coli.

Molecular Weight 重組人胸腺和激活規范趨化因子/ CCL17蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Physical Appearance 重組人胸腺和激活規范趨化因子/ CCL17蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Endotoxin Less than 1 EU/μg of rHuTARC/CCL17 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組人胸腺和激活規范趨化因子/ CCL17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人胸腺和激活規范趨化因子/ CCL17蛋白

Reference 1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308.
2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21.
3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21.
4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8.
5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.

Background Human CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16 in humans. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.

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Species	Human
Accession	Q92583
GeneID	6361
Source	Escherichia coli.
Molecular Weight	重組人胸腺和激活規范趨化因子/ CCL17蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
Physical Appearance	重組人胸腺和激活規范趨化因子/ CCL17蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuTARC/CCL17 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組人胸腺和激活規范趨化因子/ CCL17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人胸腺和激活規范趨化因子/ CCL17蛋白
Reference	1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308. 2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21. 3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21. 4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8. 5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.
Background	Human CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16 in humans. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.

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產品名稱：重組人胸腺和激活規范趨化因子/ CCL17蛋白