產品詳情
簡單介紹：
重組人類單核細胞趨化蛋白-4 / CCL13蛋白與其它公司提供的重組蛋白不同，rHuMCP-4/CCL13蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類單核細胞趨化蛋白-4 / CCL13蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類單核細胞趨化蛋白-4 / CCL13蛋白
| Synonyms | CK-beta-10, NCC-1, Small-inducible cytokine A13 |
| Species | Human |
| Accession | Q99616 |
| GeneID | 6357 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類單核細胞趨化蛋白-4 / CCL13蛋白Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人類單核細胞趨化蛋白-4 / CCL13蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMCP-4/CCL13 as determined by LAL method. |
| Reconstitution | 重組人類單核細胞趨化蛋白-4 / CCL13蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類單核細胞趨化蛋白-4 / CCL13蛋白 | |
| Reference |
1. Uguccioni M, Loetscher P, Forssmann U, et al. 1996. J Exp Med. 183:2379-84. 2. Berkhout TA, Sarau HM, Moores K, et al. 1997. J Biol Chem. 272:16404-13. 3. Garcia-Zepeda EA, Combadiere C, Rothenberg ME, et al. 1996. J Immunol. 157:5613-26. |
| Background | Human CCL13 is belonging to the CC chemokine family and is encoded by the gene CCL13 in humans. CCL13 (MCP-4) shares 56-61 % sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7) and is 60 % identical to Eotaxin (CCL11). CCL13 was a potent chemoattractant for monocytes and eosinophils and stimulated histamine release from basophils. CCL13 can induce a calcium flux in HEK-293 cells transfected with the receptor CCR2B and CCR3. That shows the function receptors of CCL3 are CCR2B and CCR3. |
