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產品詳情
  • 產品名稱:干擾素-γ/ CXCL9誘導的重組人單核因子蛋白

  • 產品型號:rHuIP-10/CXCL10
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
干擾素-γ/ CXCL9誘導的重組人單核因子蛋白與其它公司提供的重組蛋白不同,rHuIP-10/CXCL10蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.干擾素-γ/ CXCL9誘導的重組人單核因子蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

干擾素-γ/ CXCL9誘導的重組人單核因子蛋白

Synonyms Gamma-IP10, Small-inducible Cytokine B10
Species Human
Accession P02778
GeneID 3627
Source Escherichia coli.
Molecular Weight 干擾素-γ/ CXCL9誘導的重組人單核因子蛋白Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
Quantity 5μg/25μg/1000μg
AA Sequence VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 干擾素-γ/ CXCL9誘導的重組人單核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Endotoxin Less than 1 EU/μg of rHuIP-10/CXCL10 as determined by LAL method.
Reconstitution 干擾素-γ/ CXCL9誘導的重組人單核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
干擾素-γ/ CXCL9誘導的重組人單核因子蛋白
SDS-PAGE
Reference 1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6.
2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71.
3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25.
4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204.
5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62.
Background CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10.

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