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  • 產品名稱:重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白

  • 產品型號:rHuIP-10/CXCL10
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白與其它公司提供的重組蛋白不同,rHuIP-10/CXCL10蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白

Synonyms Gamma-IP10, Small-inducible Cytokine B10
Species Human
Accession P02778
GeneID 3627
Source Escherichia coli.
Molecular Weight 重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
Quantity 5μg/25μg/1000μg
AA Sequence VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Endotoxin Less than 1 EU/μg of rHuIP-10/CXCL10 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage 重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白
SDS-PAGE 重組人類γ-干擾素誘導蛋白10 / CXCL10蛋白
Reference 1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6.
2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71.
3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25.
4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204.
5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62.
Background CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10.

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