產品詳情
簡單介紹：
重組人I-TAC / CXCL11蛋白與其它公司提供的重組蛋白不同，rHuI-TAC/CXCL11蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人I-TAC / CXCL11蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人I-TAC / CXCL11蛋白
| Synonyms | Beta-R1, H174, IP-9, Small-inducible Cytokine B11 |
| Species | Human |
| Accession | O14625 |
| GeneID | 6373 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人I-TAC / CXCL11蛋白Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人I-TAC / CXCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human IL-2 activated human T-lymphocytes is in a concentration range of 0.1-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuI-TAC/CXCL11 as determined by LAL method. |
| Reconstitution | 重組人I-TAC / CXCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人I-TAC / CXCL11蛋白 | |
| SDS-PAGE |
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| Reference |
1. Tensen CP, Flier J, Rampersad SS, et al. 1999. Biochim Biophys Acta. 1446:167-72. 2. Cole KE, Strick CA, Paradis TJ, et al. 1998. J Exp Med. 187:2009-21. 3. Rani MR, Foster GR, Leung S, et al. 1996. J Biol Chem. 271:22878-84. 4. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22. |
| Background | CXCL11 also known as I-TAC is belonging to the CXC chemokine family and shares 36 % and 37 % amino acid sequence homology with IP-10 and MIG, respectively. It is highly expressed in peripheral blood leukocytes, pancreas and liver. Expression of CXCL11 is strongly induced by IFN-γ and IFN-β, and weakly induced by IFN-α. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3, which with a higher affinity than do the other chemokines for this receptor, CXCL9 and CXCL10. Similar to CXCL10, CXCL11 has been shown to be a chemoattractant for IL-2-activated T-lymphocytes, but not for isolated T-cells, neutrophils or monocytes. |
