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產品名稱：重組人沙漠Hedgehog Cys23IleIle蛋白
產品型號：rHuDHH C23II
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人沙漠Hedgehog Cys23IleIle蛋白與其它公司提供的重組蛋白不同，rHuDHH C23II蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人沙漠Hedgehog Cys23IleIle蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人沙漠Hedgehog Cys23IleIle蛋白

Species Human

Accession O43323

GeneID 50846

Source Escherichia coli.

Molecular Weight 重組人沙漠Hedgehog Cys23IleIle蛋白Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人沙漠Hedgehog Cys23IleIle蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45 μg/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 10mM PB, pH 6.0, 300mM NaCl.

Endotoxin Less than 1 EU/μg of rHuDHH C23II as determined by LAL method.

Reconstitution 重組人沙漠Hedgehog Cys23IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人沙漠Hedgehog Cys23IleIle蛋白

SDS-PAGE

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Species	Human
Accession	O43323
GeneID	50846
Source	Escherichia coli.
Molecular Weight	重組人沙漠Hedgehog Cys23IleIle蛋白Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人沙漠Hedgehog Cys23IleIle蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45 μg/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 10mM PB, pH 6.0, 300mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuDHH C23II as determined by LAL method.
Reconstitution	重組人沙漠Hedgehog Cys23IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人沙漠Hedgehog Cys23IleIle蛋白
SDS-PAGE

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產品名稱：重組人沙漠Hedgehog Cys23IleIle蛋白