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產品名稱：重組人泛素偶聯酶E2 K，His蛋白
產品型號：rHuUBE2K, His
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人泛素偶聯酶E2 K，His蛋白與其它公司提供的重組蛋白不同，rHuUBE2K, His蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人泛素偶聯酶E2 K，His蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人泛素偶聯酶E2 K，His蛋白

Synonyms HIP-2, Ubiquitin Carrier Protein, Ubiquitin-protein Ligase

Species Human

Accession P61086

GeneID 3093

Source Escherichia coli.

Molecular Weight 重組人泛素偶聯酶E2 K，His蛋白Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids (a.a.) of human UBE2K and 8 a.a. vector sequence including 6 × His tag at N-terminus.

Quantity 10μg/50μg/1000μg

AA Sequence 重組人泛素偶聯酶E2 K，His蛋白MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN

Concentration See label.

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Data is not available.

Physical Appearance 重組人泛素偶聯酶E2 K，His蛋白Sterile Colorless liquid.

Formulation A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.

Endotoxin Less than 1 EU/μg of rHuUBE2K, His as determined by LAL method.

Storage Store at -20 °C.

重組人泛素偶聯酶E2 K，His蛋白

SDS-PAGE

Reference 1. Song S, Lee H, Kam TI, et al. 2008. J Cell Biol, 182: 675-84.
2. Lee SJ, Choi JY, Sung YM, et al. 2001. FEBS Lett, 503: 61-4.
3. Song S, Kim SY, Hong YM, et al. 2003. Mol Cell, 12: 553-63.

Background Ubiquitin-conjugating enzyme E2 K belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2K gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2K has been shown to interact with Huntingtin and RNF2. Additionally, it has been implicated in the degradation of huntingtin and suppression of apoptosis.

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Synonyms	HIP-2, Ubiquitin Carrier Protein, Ubiquitin-protein Ligase
Species	Human
Accession	P61086
GeneID	3093
Source	Escherichia coli.
Molecular Weight	重組人泛素偶聯酶E2 K，His蛋白Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids (a.a.) of human UBE2K and 8 a.a. vector sequence including 6 × His tag at N-terminus.
Quantity	10μg/50μg/1000μg
AA Sequence	重組人泛素偶聯酶E2 K，His蛋白MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN
Concentration	See label.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Data is not available.
Physical Appearance	重組人泛素偶聯酶E2 K，His蛋白Sterile Colorless liquid.
Formulation	A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Endotoxin	Less than 1 EU/μg of rHuUBE2K, His as determined by LAL method.
Storage	Store at -20 °C.
重組人泛素偶聯酶E2 K，His蛋白
SDS-PAGE
Reference	1. Song S, Lee H, Kam TI, et al. 2008. J Cell Biol, 182: 675-84. 2. Lee SJ, Choi JY, Sung YM, et al. 2001. FEBS Lett, 503: 61-4. 3. Song S, Kim SY, Hong YM, et al. 2003. Mol Cell, 12: 553-63.
Background	Ubiquitin-conjugating enzyme E2 K belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2K gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2K has been shown to interact with Huntingtin and RNF2. Additionally, it has been implicated in the degradation of huntingtin and suppression of apoptosis.

產品名稱：重組人泛素偶聯酶E2 K，His蛋白