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產品名稱：重組人泛素偶聯酶E2 L3，His蛋白
產品型號：rHuUBE2L3, His
產品廠商：KALANG
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重組人泛素偶聯酶E2 L3，His蛋白與其它公司提供的重組蛋白不同，rHuUBE2L3, His蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人泛素偶聯酶E2 L3，His蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

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重組人泛素偶聯酶E2 L3，His蛋白

Synonyms L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3

Species Human

Source Escherichia coli.

Molecular Weight Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus.

Quantity 10μg/50μg/1000μg

AA Sequence 重組人泛素偶聯酶E2 L3，His蛋白MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD

Concentration See label.

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Data is not available.

Physical Appearance 重組人泛素偶聯酶E2 L3，His蛋白Sterile Colorless liquid.

Formulation A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.

Endotoxin Less than 1 EU/μg of rHuUBE2L3, His as determined by LAL method.

Storage Store at -20 °C.

重組人泛素偶聯酶E2 L3，His蛋白

Reference 1. Serniwka SAandShaw GS. 2008. Biomol NMR Assign, 2: 21-3.
2. Wenzel DM, Lissounov A, Brzovic PS, et al. 2011. Nature, 474: 105-8.
3. Whitcomb EAandTaylor A. 2009. Cell Div, 4: 17.
4. Moynihan TP, Ardley HC, Nuber U, et al. 1999. J Biol Chem, 274: 30963-8.

Background 重組人泛素偶聯酶E2 L3，His蛋白Ubiquitin-conjugating enzyme E2 L3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2L3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2L3 specifically acts with HECT-type and RBR family E3 ubiquitin-proteinshown to interact with UBOX5, ARIH1, Cbl gene, UBE3A and NEDD4. Down-regulated during the S-phase it is involved in progression through the cell cycle. Additionally, UBE2L3 regulates nuclear hormone receptors transcriptional activity and plays a role in myelopoiesis.

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Synonyms	L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3
Species	Human
Source	Escherichia coli.
Molecular Weight	Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus.
Quantity	10μg/50μg/1000μg
AA Sequence	重組人泛素偶聯酶E2 L3，His蛋白MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD
Concentration	See label.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Data is not available.
Physical Appearance	重組人泛素偶聯酶E2 L3，His蛋白Sterile Colorless liquid.
Formulation	A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Endotoxin	Less than 1 EU/μg of rHuUBE2L3, His as determined by LAL method.
Storage	Store at -20 °C.
重組人泛素偶聯酶E2 L3，His蛋白
Reference	1. Serniwka SAandShaw GS. 2008. Biomol NMR Assign, 2: 21-3. 2. Wenzel DM, Lissounov A, Brzovic PS, et al. 2011. Nature, 474: 105-8. 3. Whitcomb EAandTaylor A. 2009. Cell Div, 4: 17. 4. Moynihan TP, Ardley HC, Nuber U, et al. 1999. J Biol Chem, 274: 30963-8.
Background	重組人泛素偶聯酶E2 L3，His蛋白Ubiquitin-conjugating enzyme E2 L3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2L3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2L3 specifically acts with HECT-type and RBR family E3 ubiquitin-proteinshown to interact with UBOX5, ARIH1, Cbl gene, UBE3A and NEDD4. Down-regulated during the S-phase it is involved in progression through the cell cycle. Additionally, UBE2L3 regulates nuclear hormone receptors transcriptional activity and plays a role in myelopoiesis.

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產品名稱：重組人泛素偶聯酶E2 L3，His蛋白