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產品名稱：重組大鼠白細胞介素-22蛋白
產品型號：rRtIL-22
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠白細胞介素-22蛋白與其它公司提供的重組蛋白不同，rRtIL-22蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細胞介素-22蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

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重組大鼠白細胞介素-22蛋白

Synonyms Cytokine Zcyto 18, TIF

Species Rat

Accession Q198B4

Unigene Rn.195879.

Source Escherichia coli.

Molecular Weight 重組大鼠白細胞介素-22蛋白Approximately16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence LPINSQCKLE AANFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEELFRGV KAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSIHLSPCH ISGDDQNIQK NVRQLKETVQ KLGESGEIKA IGELDLLFMS LRNACV

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組大鼠白細胞介素-22蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRtIL-22 as determined by LAL method.

Reconstitution 重組大鼠白細胞介素-22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠白細胞介素-22蛋白

SDS-PAGE

Reference 1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9.
2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668.
3. Honda K. 2012. Immunity, 37: 952-4.
4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.

Background IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant rat Interleukin-22 contains 146 amino acids and it has approximately 79 % and 92 % amino acid sequence identity with human and murine IL-22.

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Synonyms	Cytokine Zcyto 18, TIF
Species	Rat
Accession	Q198B4
Unigene	Rn.195879.
Source	Escherichia coli.
Molecular Weight	重組大鼠白細胞介素-22蛋白Approximately16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	LPINSQCKLE AANFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEELFRGV KAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSIHLSPCH ISGDDQNIQK NVRQLKETVQ KLGESGEIKA IGELDLLFMS LRNACV
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組大鼠白細胞介素-22蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rRtIL-22 as determined by LAL method.
Reconstitution	重組大鼠白細胞介素-22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠白細胞介素-22蛋白
SDS-PAGE
Reference	1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9. 2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668. 3. Honda K. 2012. Immunity, 37: 952-4. 4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.
Background	IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant rat Interleukin-22 contains 146 amino acids and it has approximately 79 % and 92 % amino acid sequence identity with human and murine IL-22.

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產品名稱：重組大鼠白細胞介素-22蛋白